Fig. S2.

Specific binding of mProS and mGas6 to PtdSer. Nitrocellulose filters were spotted with synthetic phospholipids [phosphatidylcholine (POPC), phosphatidylserine (POPS), sphingomyelin (SM), phosphatidyldiacylglycerol (PODG), phosphatidylinositol (POPI), or phosphatidylglycerol (POPG)], or solvent (1:1 mixture of methanol and chloroform) (blank), and incubated at 4 °C overnight with 100 nM Flag-tagged mProS, mGas6, or mTim4–Fc in 3 mL of TBS containing 1% BSA and 2 mM CaCl₂. Bound proteins were detected with an HRP anti-Flag mAb or anti-human IgG Fcγ.