. 2017 Aug 2;114(33):8800–8805. doi: 10.1073/pnas.1705365114

Table 1.

Kinetic parameters for the interaction of ProS and Gas6 with TAM family proteins

TAM receptor	Analysis methods	ProS			Gas6
TAM receptor	Analysis methods	k_on × 10⁴ (M⁻¹⋅s⁻¹)	k_off × 10⁻⁴ (s⁻¹)	K_d (nM)	k_on × 10⁴ (M⁻¹⋅s⁻¹)	k_off × 10⁻⁴ (s⁻¹)	K_d (nM)
Axl	SPR	N.D.	N.D.	N.D.	37.6 ± 0.19	3.00 ± 0.01	0.80 ± 0.01
Axl	BLI	N.D.	N.D.	N.D.	77.7 ± 1.52	4.84 ± 0.08	0.62 ± 0.02
Mer	SPR	1.04 ± 0.01	6.00 ± 0.02	57.8 ± 0.58	5.47 ± 0.04	11.9 ± 0.03	21.7 ± 0.27
Mer	BLI	2.37 ± 0.02	9.39 ± 0.09	39.6 ± 0.81	11.3 ± 0.06	15.1 ± 0.09	13.4 ± 0.19
Tyro3	SPR	6.00 ± 0.05	9.70 ± 0.03	16.2 ± 0.23	10.9 ± 0.17	25.3 ± 0.12	23.3 ± 0.54
Tyro3	BLI	8.82 ± 0.12	22.0 ± 0.19	24.9 ± 0.61	21.3 ± 0.27	64.8 ± 0.32	30.5 ± 0.61

The association (k_on) and dissociation (k_off) rate constants for each ligand–receptor pair were determined by BLI (biolayer interferometry) and SPR (surface plasmon resonance). N.D., not detected.