Fig. S1.

Diagram of the microscope and the SC axes. The X, Y, and Z axes of the microscope correspond to the width (X) and length (Y) of the stage and the vertical movement of the stage during 3D image stack acquisition (Z). The x, y, and z axes of the SC correspond to the width (x), length (y), and depth (z) of the SC. Diagrammed are the various rotations that the SC can undergo with the appropriate axes labeled on top of the SC. Note that the microscope dimensions never change position, only the SC dimensions change as the SC rotates. Blue represents the LE of the SC, and pink represents the CR of the SC.