Fig. S2.

Y2H interaction of NUWA protein with cleaved DYW2. Interactions were tested using a mating-based Y2H matrix assay with AD– fusions on the horizontal axis and DNA binding domain (DB–) fusions on the vertical axis. Full-length or matured (m) DYW2 and NUWA were fused to the AD– and DB– domains. mDYW2 and mNUWA correspond to truncated proteins obtained after removal of the predicted targeting peptide. After mating, interactions were identified on –His selective medium. Among these 16 mating assays, only yeasts expressing either DB-mNUWA or DB-NUWA and AD-mDYW2 were able to grow.