Fig. S1.

Validation of the infectious human cytokine library. Lentiviruses containing control vector, IL-1β, or TNF-α were transduced to U937 monocytes, HEK293 cells with fluorescent reporter gene (NF-κB-GFP), or 293FT cells for 1 d. Light microscopy was used to record cell morphology as an indicator for cell survival of U937 monocytes following lentivirus transduction. The quantification of cell number was performed with bright-field pictures from three independent experiments by using the Cell Counter plugin of ImageJ (https://imagej.nih.gov/ij/plugins/cell-counter.html). HEK293-NF-κB-GFP cells increased GFP (green) expression in response to TNF-α and IL-1β lentivirus treatment, as shown by fluorescence microscopy. Expression of the cytokine constructs was verified by the mCherry (red) fluorescence in infected 293FT cells. Data are shown as mean + SD (n = 3). ***P < 0.001. N/V, no virus control.