| Gene | Forward, 5′-3′ | Reverse, 5′-3′ |
| ALP | ATGGGATGGGTGTCTCCACA | CCACGAAGGGGAACTTGTC |
| Collagen I | ACCGCCCTCCTGACGCAC | GCAGACGCAGATCCGGCAG |
| GAPDH | CAAGGCTGAGAACGGGAAGC | AGGGGGCAGAGATGATGACC |
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