Fig. S5.

Kinesin-2 motility properties bear similarities to kinesin-1. (A) Velocity measurements as a function of applied load (solid circles; mean ± SE; $N=49–818$) for KIF17 (red), KIF3AB (blue), and DmK1 (purple). All data were taken at 2 mM ATP. Global fits to velocity data (solid lines) are based on the four-state model. (B) RL measurements as a function of applied load (solid circles; mean ± SE; $N=49–818$) for KIF17 (red), KIF3AB (blue), and DmK1 (purple), under the same buffer conditions. Fits to assisting- and hindering-load RL measurements (solid lines) to the function $L\left(F\right)=L_0\exp \left[-\left|F\right|{\mathrm{\delta }}_L/k_{\text{B}}T\right]$ (definitions of variables are given in the main text). (C) Tabulated $L_0$ and ${\delta }_L$ parameters for both hindering- and assisting-load regimes (mean ± SE). DmK1 data and fits (56), and hindering-load and unloaded KIF3AB measurements (40), are reproduced from previous work. Velocity and RL data, as well as associated fits, are taken from Figs. 2A and 4B.