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. 2017 Aug 19;19(9):716–733. doi: 10.1016/j.neo.2017.07.001

Figure 2.

Figure 2

Amino acid sequence alignments of mouse (mJAA-F11) with humanized H2aL2a and H3L3 antibody and immunogenicity assessment of the H2aL2a and H3L3. A) Amino acid sequence alignments of mJAA-F11 heavy (top) and light (bottom) chain variable regions with variable regions of H2aL2a and H3L3 constructs. Green indicates the differences among the humanized antibodies and mJAA-F11. Alanine at position 71 (top) and Leucine at position 46 (bottom) shaded pink, indicate mouse residues that were retained to avoid steric clashes. The CDRs are shaded blue. Numbering is according to Kabat. B) Assessment of immunogenicity of the H2aL2a and H3L3 constructs by using the scoring system developed by Gao et al. [39]. T20 score is used to measure “humanness” of monoclonal antibody variable region sequences; T20 score> 80 for FR and CDR sequences is not immunogenic in humans; T20 score> 85 for FR sequences only is not immunogenic in humans (see main text for details). The closer the number is to these cut-offs the less immunogenic the antibody. FR = framework; CDR = complementarity determining region.