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. 2017 Aug 19;19(9):716–733. doi: 10.1016/j.neo.2017.07.001

Figure 5.

Figure 5

Flow cytometric analysis of TF-Ag Expression on Human Cancer cells. Flow histograms showing A) Relative TF-Ag expression levels on eight breast cancer cell lines BT549, HCC-1806, MDA-MB-453, SKBR-3, MDA-MB-468, MDA-MB-231, MDA-MB-kb2 and Du4475 detected with mJAA-F11 using rat anti-mouse IgG3-biotin/Streptavidin-PE. Solid black line is used to show the relative background with secondary antibody alone for MDA-MB-231. B) Relative TF-Ag expression levels on the breast line MDA-MB-231 compared to the lung cancer cell lines A549 and HTB-171, detected with mJAA-F11 using rat anti-mouse IgG3-biotin/Streptavidin-PE and C) binding of hJAA-F11 constructs H2aL2a and H3L3 to HTB-171 detected with Goat-anti-human IgG-biotin/Streptavidin-PE. Solid lines binding of JAA-F11 or hJAA-F11 antibody; dashed lines background binding with detection system only.