Figure 7.

ADCC activity versus TF-Ag positive breast and lung cancer cell lines by 7a) H2aL2a and chimeric antibodies at 200 μg/ml, and 7b) H3L3 and H2aL2a Ab at 50 μg/ml. A) 200 μg/ml of the H2aL2a and chimeric were used in ADCC assays against several human cancer cell lines. BC indicates a breast cancer line, while LC indicates a lung cancer line. H2aLa was most effective in ADCC against NCI-H520, then in decreasing amounts towards BT549, HTB-171, and MDA-MB-231, and the chimeric antibody showed little ADCC even at this 200 μg/ml concentration (P < .05) B) 50 μg/ml of either H2aL2a or H3L3 were used in ADCC assays against several human cancer cell lines. C) 200 μg/ml concentrations of either H2aL2a were added to BT549 cells. The mouse JAA-F11, effectively a negative control antibody did not show appreciable ADCC even at 200 μg/ml with the BT549 cells. In the HTB-171 and MDA-MB-231 cell lines with H2aL2a, there was no appreciable ADCC activity above background and was not graphed. • indicates significance differences in one representative experiment. ** indicates significance in two of three independent experiments. *** indicates significance across all three independent experiments.