Figure 12.

Immunohistochemistry to show tumor localization of H2aL2a after treatment of tumor bearing mice. The tumors from mice in the treatment study shown in Figure 11, injected with either PBS (A) or H2aL2a (B) were fixed, embedded and sectioned and stained using immunohistochemistry for the presence of human IgG in the tumor using anti-human secondary antibody. This shows the presence of H2aL2a binding to the tumor, while PBS indicates minimal background staining alone.