Table 2.

Four Similar TF-Ag Related Structures on Normal Tissues

The Glycan Array Data Showed that the Humanized hJAA-F11s and Chimeric JAA-F11 Antibodies do not Bind Galβ1–3GalNAc-Beta (β) Linked Structures and Also Common Elongation Structures on Normal Tissues. RFU Refers to Relative Fluorescence Unit.

Glycan Array #	Glycan Structure	Linker	H3L3 RFU	H2aL2a RFU	Chimeric RFU
a144	Galβ1–3GalNAcβ1-4(Neu5Acα2–3)Galβ1 4Glcβ-	Sp0	10	7	-1
b145	Galβ1-3GalNAcβ1-4Galβ1-4Glcβ-	Sp8	15	29	28
c88	GlcNAcβ1-3Galβ1-3GalNAcα-	Sp8	19	49	78
d223	Neu5Acα2-3Galβ1-3GalNAcα-	Sp8	11	22	109

All RFU on these saccharides are <1% of the reaction with the TF-Ag alpha disaccharide-Sp8.

^aGlycan #144 is ceramide ganglioside GM1 found on brain and red blood cells.

^bGlycan #145 is ceramide asialo-GM1 found on NK cells, kidneys, spleen, and regenerating respiratory epithelial cells.

^cGlycans # 88 and #223 are common elongation structures on normal tissues.

^dGlycans # 88 and #223 are common elongation structures on normal tissues.