Figure 3.

Conditional Deletion of Nkx2-1 in the Developing Septum and Changes in Gene Expression

(A) Breeding strategy for conditional deletion of Nkx2-1 in septal precursors.

(B) Neuroepithelial changes in gene expression in septal conditional mutant embryos (Zic4-Cre;Nkx2-1^fl/fl) compared to controls (Zic4-Cre;Nkx2-1^fl/+) at E12.5. ISH (blue signal), IHC (black signal). The loss of Nkx2-1 mRNA and protein, reduction of Gsh2, and upregulation of Nkx6-2 and Gli1 (arrows) observed in the mutants are shown.

(C) Gene expression changes in postmitotic neurons of the septum. ISH (blue), IHC (black). The loss of NKX2-1 and reduction of Lhx6 and Lhx7 expression observed in mutants are shown.

Scale bars: 100 μm in (B) and 50 μm in (C).