Figure 5.

Loss of Septal NKX2-1 Causes Reduction of Forebrain Cholinergic Neurons

(A–D) Reduction in CHAT^+ve neurons in controls and mutants at P90 in the rostral striatum (STR) (A), the horizontal diagonal band (hDB) (B), the preoptic nucleus (MCPO) (C), the globus pallidus (GP), and substantia innominata (SI) (D).

(E) Quantification of CHAT^+ve neurons in the forebrain of controls and mutants at P90 (mean ± SEM; n = 4). Unpaired two-tailed t test: ^∗p < 0.05 and ^∗∗∗p < 0.0001.

(F) Quantification of CHAT^+ve neurons throughout the rostro-caudal extent of the striatum (mean ± SEM). Two-way RM ANOVA. Post hoc uncorrected Fisher’s LSD, levels 1–9, ^∗∗∗p < 0.0001; level 10, ^∗∗p < 0.01; and levels 11–15, p > 0.05.

(G) Gantt chart showing the levels of count for each region measured as the progressive numbering of sections from rostral to caudal levels.

R-C, rostro-caudal. Scale bars: left 250 μm, right 50 μm in (A); 100 μm in (B); and 200 μm in (C) and (D).