Table 2.

Medium compositions for sorghum P898012 experiments

Medium name and number	Medium name	Media components
Sb-1	Inoculation	MS salts 2.15 g/L, sucrose 68.5 g/L, glucose 36.0 g/L, 2, 4-D 1.5 mg/L, casamino acids 1 g/L, B5 vitamins (1000×) 1 ml, pH 5.2 and acetosyringone 1 ml from 100 mM concentration
Sb-2	Co-cultivation	MS salts 2.15 g/L, sucrose 20 g/L, glucose 10 g/L, l-proline 700 mg/L, 2,4-D 2.0 mg/L, MES 500 mg/L, ascorbic acid 10 mg/L, B5 vitamins (1000×) 1 ml, pH 5.8, polyvinylpolypyrrolidone (PVPP) 10 g/L, Agar 8 g/L, and acetosyringone 1-ml from 100-mM concentration
Sb-3	Resting	MS salts 4.33 g/L, sucrose 30 g/L, 2,4-D 2.0 mg/L, MES 500 mg/L, ascorbic acid 10 mg/L, asparagine 150 mg/L, coconut water 100 mL/L, B5 vitamins (1000×) 1 mL/L, pH 5.8, phytagel 2.5 g/L, PVPP 10 g/L, *carbenicillin 200 mg/L, *timentin 150-mg/L* prepared fresh and added after autoclave
S4	Callus induction	MS salts 4.33 g/L, sucrose 30 g/L, MES 500 mg/L, 2,4-D 1.5 mg/L, B5 vitamins (1000×) 1 mL/L, pH 5.8, phytagel 2.5 g/L, PVPP 10 g/L, *carbenicillin 200 mg/L, *timentin 150-mg/L* prepared fresh and added after autoclave
Sb-5	Desiccation	2 Sterile, dry Whatman #70 mm filter paper sealed with parafilm
Sb-6	Embryo Proliferation	MS salts 4.33 g/L, sucrose 30 g/L, MES 500 mg/L, 2,4-D 1.5 mg/L, kinetin 0.5 mg/L, B5 vitamins (1000×) 1 mL/L, pH 5.8, phytagel 2.5 g/L, PVPP 10 g/L, *carbenicillin 200 mg/L, *timentin 150 mg/L* prepared fresh and added after autoclave
Sb-7	Regeneration	MS salts 4.33 g/L, sucrose 60 g/L, MES 500 mg/L, l-proline 700 mg/L, zeatin 0.5 mg/L, IAA 1 mg/L, ABA 0.025 mg/L, TDZ 0.1 mg/L, MS vitamin 5 mL/L (1000×), Myo-inositol 100 mg/L, pH 5.8, agar 8 g/L, PVPP 10 g/L, *carbenicillin 200 mg/L, *timentin 150 mg/L* prepared fresh and added after autoclave
Sb-8	Rooting	MS salts 2.15 g/L, sucrose 30 g/L, NAA 0.25 mg/L, IBA 0.25 mg/L, MS vitamin (1000×) 1 mL/L, pH 5.8, phytagel 2.5 g/L, *carbenicillin 200 mg/L, *timentin 150-mg/L* prepared fresh and added after autoclave