Table 3.

Transient GFP expression in maize B73 after 3 days of co-cultivation and embryogenic calli recovery with Agrobacterium AGL1, AGL1 with PHP78891, or AGL1 with PHP78891 and super-binary vector (All the GFP transient foci are developed into embryogenic callus or embryoids)

Experiment	Agrobacterium and constructs	No. of immature embryos infected	GFP transient expression	% of transient expression	No. of embryogenic calli	% of embryogenic calli
1	AGL1	75	0	0	0	0
2	AGL1	70	0	0	0	0
3	AGL1	80	0	0	0	0
1	AGL1 PHP78891	60	41	68.3	41	68.3
2	AGL1 PHP78891	50	36	72	36	72
3	AGL1 PHP78891	65	43	66.1	43	66.1
1	AGL1 PHP78891-SBV	55	53	96.3	53	96.3
2	AGL1 PHP78891-SBV	60	59	98.3	59	98.3
3	AGL1 PHP78891-SBV	80	78	97.5	78	97.5