Table 5.
Frequency of GFP expression of PHP78891 without selection using Agrobacterium EHA101 in sorghum P898012
Experiment | Agrobacterium EHA101 with or without PHP78891 | No. of immature embryos infected | GFP positive embryogenic calli | % GFP expression |
---|---|---|---|---|
1 | EHA101 PJLU13 | 11 | 0 | 0 |
2 | EHA 101 PYU 2593 | 55 | 0 | 0 |
3 | EHA 101 PYU 2593 | 46 | 0 | 0 |
4 | EHA101 PHP78891 | 11 | 6 | 54.54 |
5 | EHA101 PHP78891 | 180 | 28 | 15.56 |
6 | EHA101 PHP78891 | 128 | 30 | 23.44 |
7 | EHA101 PHP78891 | 52 | 10 | 19.23 |