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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Chem Neuroanat. 2017 May 30;83-84:91–98. doi: 10.1016/j.jchemneu.2017.05.003

Figure 3. Pseudophosphorylation of DAT N-terminal serines bypasses the requirement for raft localization in the actions of amphetamine.

Figure 3

A) Cells stably expressing FLAG DAT were incubated with or without 25 μg/mL nystatin for 20 min and then treated for 15 min with 0.5 μM PMA or vehicle. DAT was immunoprecipitated by the FLAG tag and probed for phosphorylated serine 7 (pSer7) or total DAT. B) Larvae expressing UAS-driven small RNA-mediated interference against both Drosophila DAT (dDATi) and Flot1 (dFlot1i) in dopamine neurons using a Gal4 driver specific to tyrosine hydroxylase (TH)-positive neurons (TH-GAL4) exhibited a blunted AMPH response that was not rescued by expression of hDAT. In contrast, expression of hDAT/S to D did rescue the response to AMPH (***P<0.001). Data = mean±S.E.M. (n ≥ 30 larvae per group).