Skip to main content
. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Gut. 2017 Feb 21;67(5):827–836. doi: 10.1136/gutjnl-2016-312940

Figure 2. Increased inflammation in ENS microenvironment with age.

Figure 2

Heat map of cytokine and chemokine expression of LMMP lysate from young and old mice from Luminex analysis revealed higher pro-inflammatory cytokines and chemokines with age (A). Elevation of IL-6 was seen on a second cohort of young and old mice by Western blot, which was statistically significant based on pixel intensity normalized to GAPDH (B). Flow cytometry performed on enzymatically dissociated LMMP with gating of live, single cells revealed increased numbers of CD45+ leukocytes (as percent of live cells) in old mice compared to young (C). There was also a statistically significant rise in lymphocytes (CD3+CD19+) in old compared to young mice but no difference in dendritic cells (CD11c+ F4/80−) as a percent of live cells (D). n≥5 for each group; *p<.05, **p<.01 by Mann Whitney for (A); **p<.01 by t-test for (B); *p<.05, **p<.01 by one-way ANOVA with Bonferroni’s multiple comparisons test for (C) and (D).