Fig. 4.
Dose-response curves for the most active diglycosyl diselenides. A) Bloodstream T. b. brucei and B) murine macrophages (cell line J774) were treated with different concentrations of 13 and 15. After 24 h incubation, cell viability was assessed by flow cytometry (Accuri BD) using propidium iodide as exclusion dye. The data were fitted to the Hill equation with R2 values > 0.96. The EC50 against T. b. brucei is 0.54 ± 0.05 μM for 13 and 1.49 ± 0.01 μM for 15, contrasted to the values of 4.9 ± 0.45 μM for 13 and 6.9 ± 0.54 μM for 15 against macrophages.