Specifications
Organism/cell line/tissue	Wild soybean (Glycine soja Sieb. & Zucc.)/whole root tissue
Sex	Not applicable
Sequencer or array type	Illumina HiSeq 2500
Data format	Raw reads in fastq.gz format
Experimental factors	Seedling roots were treated with infective second-stage juveniles (J2s) of the soybean cyst nematode (HG type 2.5.7) for 3, 5, 8 days. Roots from four individual plants were pooled as one biological replicate, and three replicates were prepared for treatment and control at each time point.
Experimental features	This study uses wild soybean that contains higher levels of genetic diversity compared with cultivated soybean as a study system. Two wild soybean genotypes with distinct response to SCN were used for comparative analyses.
Consent	Not applicable
Sample source location	Samples were originally collected from East Asia. Seeds could be requested at USDA Soybean Germplasm Collection, United States (http://www.ars-grin.gov/).