Figure 7.

Functional redundancy analysis of control and MAP1B-deficient neurons. Quantitative fluorescence measurements of tau (A) and MAP2 (B) immunolabeling from cultured hippocampal pyramidal neurons of wild-type or MAP1B-deficient neurons. Measurements were performed in cells fixed prior or after detergent extraction performed under microtubule-stabilizing conditions. Regions are equivalent to those of previous figures. Groups: ▪, wild-type neurons fixed before extraction; ▨, wild-type neurons fixed after extraction; □, MAP1B-deficient neurons fixed before extraction; and ▤, MAP1B-deficient neurons fixed after extraction. A total of 75 cells was analyzed for each experimental condition. (C–F) Double fluorescence micrographs of cytoskeletal preparations showing β-tubulin (C and E) and MAP2 (D and F) immunolabeling at the distal axonal third of wild-type (C and D) or MAP1B-deficient (E and F) neurons. Both axons were of equivalent length. Bar, 5 μm.