Figure 5.

CCL19 and CCL21 enhance osteoclast bone resorption activity. (a) BMMs were placed on calcium phosphate-coated plates and cultured with M-CSF (30 ng ml⁻¹) and RANKL (100 ng ml⁻¹), along with CCL19 (10 ng ml⁻¹) or CCL21 (10 ng ml⁻¹). After 6 days, calcium phosphate was stained with von Kossa reagents, and the resorbed area was quantified. (b) BMMs were placed on dentin slices and cultured with M-CSF (30 ng ml⁻¹) and RANKL (100 ng ml⁻¹), along with CCL19 (10 ng ml⁻¹) or CCL21 (10 ng ml⁻¹). After 6 days, the dentin slice surfaces were analyzed by confocal microscopy. The dentin slices were stained with trypan blue, and the resorption pit areas were quantified. (c) BMMs were cultured on cover glasses and cultured with M-CSF (30 ng ml⁻¹) and RANKL (100 ng ml⁻¹) in the presence of CCL19 (10 ng ml⁻¹) or CCL21 (10 ng ml⁻¹). After 6 days, the cells were fixed and incubated with rhodamine phalloidin. The actin ring density was quantified by confocal microscopy. BMM, bone marrow-derived macrophages.