Figure 8.

CCL19 and CCL21 stimulate bone resorption in vivo. (a) Collagen sponges soaked with CCL19 (2 μg) or PBS were transplanted into 5-week-old ICR mouse calvariae. LPS or PBS was injected into the mice intraperitoneally 1 day before and 3 days after transplantation. At 7 days after transplantation, the calvariae were fixed with 4% paraformaldehyde and analyzed by μ-CT. The decalcified calvarial tissue sections were stained for TRAP, and the osteoclast surface/bone surface index was assessed using the Osteomeasure program. (b) Collagen sponges soaked with CCL21 (2 μg) or PBS were transplanted into 5-week-old ICR mouse calvariae. After 7 days, the calvariae were fixed with 4% paraformaldehyde for 1 day and analyzed by μ-CT. The osteoclast surface/bone surface index was determined with decalcified calvarial sections after TRAP-staining using the Osteomeasure program. LPS, lipopolysaccharide; μ-CT, micro-computed tomography.