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Construction of pSG3-based aph lacZ fusions in S. Typhimurium fimbrial operons. Promoters were isolated as PCR products ranging from 271 to 391 bp and cloned into plasmid pSG3 to construct chromosomal fusions of each fimbrial operon promoter to aph. The resulting promoter fusions are illustrated. Strains are resistant to kanamycin only when the corresponding fimbrial promoter is active.
