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. 2017 Aug 22;8(4):e01189-17. doi: 10.1128/mBio.01189-17

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Copyright © 2017 Łaniewski et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Serum IgG1 and IgG2a responses to PspA in mice immunized with RASV strains expressing fimbriae in a constitutive manner (Saf⁺, Sti⁺, Stc⁺, and Agf⁺) and producing PspA antigen. The kinetics of serum IgG1 and IgG2a responses to PspA in mice are shown. The data represent the concentrations of anti-PspA IgG1 (A) and IgG2a (B) in pooled serum samples from eight mice measured in duplicate. Error bars show the differences between the duplicates (standard deviations). All samples from immunized mice were significantly different from those from the control group given PBS (P < 0.05). Values that are significantly different from the values for the S. Typhimurium χ11606(pYA4088) group are indicated by asterisks as follows: *, P < 0.05; **, P < 0.01, ****, P < 0.0001. (C) Calculated IgG2a/IgG1 ratios based on the data shown in panels A and B.