Progression of LGTV envelope protein synthesis in infected organ cultures. Magnification, ×20 (LGTV-infected MG, salivary gland [SG], and synganglion [SN]). md, midgut diverticulum; ld, lobular duct; a.c., acinus; pg/h, periganglionic sinus/sheath; n.p., neuropile; rca, retrocerebral area; mgt, midgut tissue. LGTV envelope protein within tissue is denoted by the prominent purple color. Mock-infected organs at 96 hpi were processed and stained in the same manner with LGTV antibody to identify any potential nonspecific staining background (see Fig. S3).