FIG 9 .

dsRNA transfection effect on infectious LGTV and POWV replication from infected midgut and salivary gland cultures. pGEM, pGEM plasmid (negative control); LGTV 3UTR, 3′ UTR of LGTV strain TP21. Transfection of LGTV-infected midgut (A), POWV-infected midgut (B), LGTV-infected salivary gland pair (C), and POWV-infected salivary gland pair (D) with 10 ng pGEM dsRNA and LGTV 3′ UTR dsRNA. Transfections of midguts and salivary glands were completed for 108 and 180 h, respectively, before supernatants were collected for immunofocus assays, which were normalized to the pGEM dsRNA negative-control response. An unpaired t test was completed to compare FFU per milliliter of the LGTV 3′ UTR dsRNA to that for the pGEM negative control. Error bars represent standard errors of the means, and data are representative of two technical replicates for each sample for the immunofocus assays of two biological replicates. **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.