FIG 2 .

dsRNA depletion of transcripts encoding proteins involved in translation and the host cell cycle restrict L. pneumophila intracellular growth. Intracellular growth was determined by automated image analysis (Materials and Methods). D. melanogaster cells were treated with dsRNA directed against transcripts encoding translation factors (A) or cell cycle regulators (B) for 4 days and challenged with L. pneumophila GFP⁺ cells at an MOI of 1. Intracellular growth was measured at various times after challenge by calculating the total area of Legionella vacuoles relative to the area of cell nuclei. Numbers within parentheses denote the use of different dsRNAs for the same target gene.