FIG 3 .

Cell cycle analysis of dsRNA-depleted Kc167 cells shows cell cycle disruption. (A) dsRNA-treated cells were challenged with Lp01 lux⁺ at an MOI of 1. Intracellular replication was measured 45 h after challenge. (B) D. melanogaster cells were treated with dsRNA against translation components (top panel) and the cell cycle control (bottom panel), followed by staining with 1 µg/ml Hoechst stain to determine DNA content using flow cytometry (Materials and Methods). **, P < 0.01, and ***, P < 0.001, compared to untreated cells by one-way analysis of variance (ANOVA) with post hoc Dunnett’s test.