Figure 6.
Effect of aphidicolin and UV light on the subnuclear distribution of hLigI. Exponentially growing HeLa cells were stained after the indicated treatments with 2B1 mAb and with FITC-conjugated goat anti-mouse IgG secondary antibody. a, untreated cells; b, cells stained after 3 h from UV irradiation at 20 J/m2; c, cells kept for 3 h in 2 μg/ml aphidicolin; d, cells treated for 3 h with 100 μM VP-16; e, cells grown for 3 h in 2 μg/ml aphidicolin and 100 μM VP-16. Arrows point to cells in which hLigI has a typical mid-S-phase pattern.