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. 2017 Apr 14;24(2):68–77. doi: 10.21315/mjms2017.24.2.9

Table 1.

Primers used for VP7 genotyping of rotavirus strains

Primer Sequence ( 5′-3′) Position PCR prouct (bp) References
First amplification of G-typing as described by Das et al. (13) and Gomara et al. (15)
9con1L TAG CTC CTT TTA ATG TAT GGT AT 37–59 895 Das BK et al. (13)
VP7R AAC TTG CCA CCA TTT TTT CC 914–932 Gomara MI et al. (15)
First amplification of G-typing as described by Gomara et al. (15)
VP7F ATG TAT GGT ATT GAA TAT ACC AC 51–71 881 Gomara MI et al. (15)
VP7R AAC TTG CCA CCA TTT TTT CC 914–932 Gomara MI et al. (15)
Second amplification of G-typing as described by Das et al. (13)
G1 9T-1 TCT TGT CAA AGC AAA TAA TG 176–195 158 Das BK et al. (13)
G2 9T-2 GTT AGA AAT GAT TCT CCA CT 262–281 244 Das BK et al. (13)
G3 9T-3P GTC CAG TTG CAG TGT AGC 484–501 464 Das BK et al. (13)
G4 9T-4 GGG TCG ATG GAA AAT TCT 423–440 403 Das BK et al. (13)
G9 9T-9B TAT AAA GTC CAT TGC AC 131–147 110 Das BK et al. (13)
Second amplification of G-typing as described by Gouvea et al. (16)
G1 aBT1 CAA GTA CTC AAA TCA ATG ATG G 314–335 618 Gouvea et al. (16)
G2 aCT2 CAA TGA TAT TAA CAC ATT TTC TGT G 411–435 521 Gouvea et al. (16)
G3 G3 ACG AAC TCA ACA CGA GAG G 250–259 682 Gouvea et al. (16)
G4 aDT4 CGT TTC TGG TGA GGA GTT G 480–498 452 Gouvea et al. (16)
G8 aAT8 GTC ACA CCA TTT GTA AAT TCG 178–198 754 Gouvea et al. (16)
G9 aFT9 CTT GAT GTG ACT AYA AAT AC 757–776 179 Gouvea et al. (16)