Table 2.
Primer | Sequence (5′-3′) | Position | PCR product (bp) | References | |
---|---|---|---|---|---|
First amplification of P-typing as described by Gentsch et al. (14) | |||||
Con-3 | TGG CTT CGC TCA TTT ATA GAC A | 11–32 | 876 | Gentsch et al. (14) | |
Con-2 | ATT TCG GAC CAT TTA TAA CC | 868–887 | Gentsch et al. (14) | ||
First amplification of P-typing as described by Simmonds et al. (8) | |||||
VP4F | TAT GCT CCA GTN AAT TGG | 132–149 | 663 | Simmonds et al. (8) | |
VP4R | ATT GCA TTT CTT TCC ATA ATG | 775–795 | Simmonds et al. (8) | ||
Second amplification of P-typing as described by Gentsch et al. (14) | |||||
P[4] | 2T-1 | CTA TTG TTA GAG GTT AGA GTC | 474–494 | 483 | Gentsch et al. (14) |
P[6] | 3T-1 | TGT TGA TTA GTT GGA TTC AA | 259–278 | 267 | Gentsch et al. (14) |
P[8] | 1T-1D | TCT ACT TGG ATA ACG TGC | 339–356 | 345 | Gentsch et al. (14) |
P[9] | 4T-1 | TGA GAC ATG CAA TTG GAC | 385–402 | 391 | Gentsch et al. (14) |
P[10] | 5T-1 | ATC ATA GTT AGT AGT CGG | 575–594 | 583 | Gentsch et al. (14) |