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. 2017 Apr 14;24(2):68–77. doi: 10.21315/mjms2017.24.2.9

Table 2.

Primers used for VP4 genotyping of rotavirus strains

Primer Sequence (5′-3′) Position PCR product (bp) References
First amplification of P-typing as described by Gentsch et al. (14)
Con-3 TGG CTT CGC TCA TTT ATA GAC A 11–32 876 Gentsch et al. (14)
Con-2 ATT TCG GAC CAT TTA TAA CC 868–887 Gentsch et al. (14)
First amplification of P-typing as described by Simmonds et al. (8)
VP4F TAT GCT CCA GTN AAT TGG 132–149 663 Simmonds et al. (8)
VP4R ATT GCA TTT CTT TCC ATA ATG 775–795 Simmonds et al. (8)
Second amplification of P-typing as described by Gentsch et al. (14)
P[4] 2T-1 CTA TTG TTA GAG GTT AGA GTC 474–494 483 Gentsch et al. (14)
P[6] 3T-1 TGT TGA TTA GTT GGA TTC AA 259–278 267 Gentsch et al. (14)
P[8] 1T-1D TCT ACT TGG ATA ACG TGC 339–356 345 Gentsch et al. (14)
P[9] 4T-1 TGA GAC ATG CAA TTG GAC 385–402 391 Gentsch et al. (14)
P[10] 5T-1 ATC ATA GTT AGT AGT CGG 575–594 583 Gentsch et al. (14)