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Equilibrium dissociation constant (Kd) of select aptamers in LX-2 and HSC. Apparent Kd values of the DNA aptamers annealed with Alexa-488 labeled oligonucleotides in LX-2 (A) and HSC-T6 (C) cells. Apparent Kd values of the DNA aptamers labeled Alexa-488 in LX-2 (B) and HSC-T6 (D). The cells were incubated with the aptamers at different concentrations at 37℃ for 1 h. The labeled cells were determined using flow cytometry, and apparent Kd values were calculated using GraphPad.
