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Knockdown of IGFIIR decreases the affinity of aptamer-20 to LX-2 cells. LX-2 cells were transfected with 50 nM of scramble siRNA or IGFIIR siRNA for 48 h. The expression of IGFIIR was determined by western blot (A). After knockdown of IGFIIR, the cells were incubated with aptamer-20 at 37℃ for 1 h, and the labeled cells were counted using flow cytometry (B). Data are presented as mean ± SD (n=3).
