Figure 2.

The role of lncRNA-LET in GEM-induced cancer stemness. (A) qRT-PCR showed stable overexpression of lncRNA-LET in T24 cells (pLET). (B) CSC markers were determined by Western blotting in T24 cells overexpressed lncRNA-LET and control vector. (C-E) Tumor size change (C), the levels of lncRNA-LET (D) and representative IHC (E) of xenografts from control (Vec) and overexpression (pLET) cells after GEM or vehicle treatment (Vec+Veh group n=5, other group n=6). Dashed boxes indicate the time frame of each GEM chemotherapy cycle. Data are shown as mean ± SD and represent at least two independent experiments with similar results. * P < 0.05, ** P < 0.01, *** P < 0.001 (One-way ANOVA followed by Newman-Keuls multiple comparison test).