Figure 4.

RBFOX3 regulated HCC cell migration and invasion in vivo and in vitro via hTERT signaling pathway. (A) The relative cell migration and invasion ratio were increased in RBFOX3 overexpressed cells compared with vector overexpressed cells in QGY7703 cells. (B) The relative cell migration and invasion ratio were effectively decreased in RBFOX3 knockdown Hep3B cells. (C) MMP9 and MMP2 protein level were detected and quantified in RBFOX3 or vector overexpressed QGY7703 cells. (D) MMP9 and MMP2 protein level were detected and quantified in sh-RBFOX3 or sh-NC treated Hep3B. (E) Overexpression of hTERT reversed the inhibition of cell migration and invasion by RBFOX3 knockdown in QGY7703 cells. (F) Overexpression of hTERT reversed the inhibition of cell migration and invasion by RBFOX3 knockdown in Hep3B cells. (G) Arrows showed the representative results of metastatic lung nodules, and H&E staining was used to stain metastatic lung nodules (upper panels, scale: 40×; lower panel, scale: 100×). The right panel illustrated the statistical results (n=5). (H) Arrows showed the representative results of metastatic lung nodules, and H&E staining was used to stain metastatic lung nodules (upper panel, scale: 40×; lower panel, scale: 100×). The right panels illustrated the statistical results (n=5). The cell migration capacity was analyzed by scratch assay and the cell invasion capacity was determined by trans-well assay. Data were shown as means ± SD. n = 5 for each group. *p<0.05 by two-tailed Student's t-test.