Table 1.
Micr1 | MIC (mg/ml) | ||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Bucida buceras | Breonadia salicina | Harpephyllum caffrum | Olinia ventosa | Vangueria infausta | Xylotheca kraussiana | Amp | |||||||||||||||||||||
Time2 (h) | Extractant3 A |
H | D | M | A | H | D | M | A | H | D | M | A | H | D | M | A | H | D | M | A | H | D | M | Ave | ||
A. p | 24 | 0.63 | 1.25 | 1.25 | 1.25 | 0.63 | 2.5 0 | 0.63 | 1.25 | 1.25 | 2.50 | 1.25 | 0.63 | 1.25 | 1.25 | 0.63 | 1.25 | 1.25 | 2.50 | 0.63 | 0.63 | 0.16 | 0.63 | 0.32 | 0.32 | 1.06 | 0.02 |
A. n | 48 | 0.63 | 2.50 | 2.50 | 1.25 | 2.50 | 1.2 5 | 1.25 | 2.50 | 1.25 | 2.50 | 1.25 | 0.63 | 1.25 | 2.50 | 1.25 | 2.50 | 1.25 | 2.50 | 2.50 | 2.50 | 0.63 | 2.50 | 1.25 | 1.25 | 1.63 | 0.02 |
C. g | 48 | 0.63 | 2.50 | 2.50 | 0.63 | 1.25 | 2.5 0 | 1.25 | 1.25 | 1.25 | 0.63 | 1.25 | 2.50 | 1.25 | 2.50 | 1.25 | 2.50 | 1.25 | 2.50 | 2.50 | 2.50 | 1.25 | 2.50 | 0.63 | 0.63 | 1.54 | <0.0 2 |
P. e | 24 | 0.08 | 0.63 | 0.32 | 0.32 | 1.25 | 2.5 0 | 2.50 | 2.50 | 2.50 | 1.25 | 2.50 | 0.63 | 0.32 | 1.25 | 1.25 | 0.63 | 2.50 | 1.25 | 1.25 | 1.25 | 0.63 | 2.50 | 1.25 | 1.25 | 1.27 | <0.0 2 |
P. j | 48 | 0.02 | 0.08 | 0.32 | 0.02 | 0.08 | 0.0 8 | 0.08 | 0.08 | 0.04 | 0.32 | 0.16 | 0.08 | 0.08 | 0.32 | 0.16 | 0.32 | 0.63 | 0.63 | 0.63 | 0.32 | 0.32 | 0.63 | 0.63 | 0.63 | 0.28 | <0.0 2 |
T. h | 48 | 0.02 | 0.08 | 0.63 | 0.02 | 0.63 | 0.3 2 | 0.63 | 0.63 | 0.08 | 0.63 | 0.63 | 0.08 | 0.04 | 0.04 | 0.04 | 0.08 | 0.32 | 0.32 | 0.32 | 0.32 | 0.16 | 0.32 | 0.16 | 0.32 | 0.28 | <0.0 2 |
F. o | 24 | 0.02 | 0.63 | 0.32 | 0.04 | 0.32 | 0.0 8 | 0.16 | 0.16 | 0.02 | 0.32 | 0.16 | 0.04 | 0.08 | 0.63 | 0.32 | 0.16 | 0.32 | 0.63 | 0.32 | 0.32 | 0.63 | 0.32 | 0.32 | 0.32 | 0.28 | <0.0 2 |
Ave | 0.29 | 1.02 | 1.05 | 0.5 | 0.92 | 1.2 5 | 0.89 | 1.12 | 0.88 | 1.09 | 0.99 | 0.62 | 0.61 | 1.14 | 0.70 | 0.99 | 1.04 | 1.37 | 1.09 | 1.05 | 0.54 | 1.23 | 0.65 | 0.67 | 0.91 |
Microorganisms: A. p. = Aspergillus parasiticus, A. n. = Aspergillus niger, C. g. = Colletotrichum gloeosporioides, P. e. = Penicillium expansum, P. j. = Penicillium janthinellum, T. h. = Trichoderma harzianum and F. s. = Fusarium oxysporum.
Time: MIC values after 24 h were sometimes not distinct, so the plates were left to incubate for a further 24 h before MIC was read
Extractant: A = acetone, H = hexane, D = dichloromethane, M = methanol