Table 4.

Summary of labeling strategies commonly used in super-resolution microscopy

The switching buffers section of the table includes a list of working dye/buffer combinations. Example buffer compilation: decide on buffer class (e.g., “Reducer with –O₂”), and then decide on the buffer base, the pH, oxygen removal (−O₂), and the final compounds based on the fluorophore used (e.g., CF 680 with 10–100 mM MEA [63])

AF Alexa Fluor, –O₂ oxygen removal, for the abbreviations of the chemicals, see the text or the corresponding references

* For the exact formulations of GLOX, PCD/PCA, and PCO, see the corresponding references