Figure 6.

ApmapE1-KO show improved metabolic phenotype on an HFD. A) Blood glucose of WT and ApmapE1-KO mice measured in fed ad libitum and overnight-unfed (“fast”) state after 12 wk of HFD; n = 6. B) Respiratory exchange ratio (RER) was measured after 2 d of adaption period for further 2.5 d in metabolic cages; n = 6. C) Energy expenditure (EE) was calculated from Vco₂ and Vo₂ measured in metabolic cages; n = 6. D) Physical activity was assessed in metabolic cages; n = 6. E) Glucose tolerance (1.5 g glucose/kg body weight) and insulin tolerance tests (0.5 U insulin/kg body weight; F) were performed in male WT and ApmapE1-KO mice after 12 and 8 wk of HFD, respectively; n = 4 for GTT, n = 10 for ITT. Statistics calculated with 2-way ANOVA with Bonferroni post hoc test using GraphPad Prism software. F) Inlet plasma insulin levels before and after glucose bolus. G, H) mRNA expression of inflammatory markers (G) and collagens (H) in eWAT of WT and ApmapE1-KO mice (22 wk of HFD); n ≥ 4. I) Total collagen in eWAT was measured after 22 wk of HFD; n = 8–10. J) Trichrome-stained sections of eWAT from ApmapE1-KO and WT mice after 20 wk of HFD. Maximal fibrosis/collagen staining of each group is shown. One representative replicate is shown. Scale bars: 500 µm (left), 200 µm (right); n = 4. *P ≤ 0.05, **P ≤ 0.01 (Student’s t test).