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. 2017 May 30;31(9):4088–4103. doi: 10.1096/fj.201601337R

Figure 7.

Figure 7.

APMAP interacts with LOXL1 and 3 and influences their expression level. A) Protein expression of LOXL3 in eWAT of HFD-fed ApmapE1-KO and WT mice; n = 3–4. B) mRNA expression of Lox, Loxl1, and Loxl3 in eWAT; n ≥ 4. C–E) Interaction of APMAP_E1 with LOXL1 (C), LOXL3 (D), and LOX (E) in COS7 cells. Pulldown was performed with flag beads. COS7 cells were transfected with Loxl1/-3-flag or Apmap-flag vectors and His-Apmap or His-Lox vector. Empty pCMV 5.1 flag and His-LacZ served as controls. One representative pulldown of n ≥ 3 is shown. F) Scheme of APMAP full-length and truncated protein variants. TM, transmembrane region. G, H) Flag beads pulldown with LOXL1-flag (G) and LOXL3-flag (H) and APMAP_E1 and truncated variants (E2, -3, -6) in COS7 cells. *P ≤ 0.05, **P ≤ 0.01 (Student’s t test).