TABLE 1.
Murine primer sequences used for qPCR
| Primer,
5′−3′ |
||
|---|---|---|
| Target gene | Forward | Reverse |
| Apmap E7/8 | GAAGACTTTGTCCTAGTGGCAG | ATTGTCAGGAAATCCAGGCATG |
| Apmap_E1 | GAGTGTCAAGGCGCTGTTTGG | GGCCATCGTCCGTGACGAC |
| Apmap_E2 | GCTTGTCAGTGTGTGTGGCTC | CTGAAACTCTGAGGATCTATGG |
| Apmap_E3 | GTTGAACTTGGGCTGGTTATAG | TTCTGCTTGCCGCAACTTCG |
| Apmap_E6 | CATGGTGACTGTTTAGGGAGG | TAGGTTCATCATCTCGGGTTTC |
| Cebpα | ATCTGCGAGCACGAGACGTC | TGTCGGCTGTGCTGGAAGA |
| Cidea | TGACATTCATGGGATTGCAGAC | GGCCAGTTGTGATGACTAAGAC |
| Col1a1 | ATGGATTCCCGTTCGAGTACG | TCGGTGGACATTAGGCGC |
| Col3a1 | CTCTTATTTTGGCACAGCAGTC | ATTTGACATGGTTCTGGCTTCC |
| Col4a1 | GTGTGCATGAGAAGAACATAAC | TTCTAGGGTTCATTGCTGTTAC |
| Col6a1 | CACACATACCGGCGCAATT | TCTGGCAGCCTGGCACTC |
| Col6a2 | ATCGCTAACTCTCCACATGAGCTC | AGCTCACCTTGTAGCACTCTCCA |
| Col6a3 | ATCAACCTCATGGTGAACACAG | TCTCTAGGTCATAGTGCCATAG |
| Emr1 | CTTTGGCTATGGGCTTCCAGTC | GCAAGGAGGACAGAGTTTATCGTG |
| Il-1b | GAAATGCCACCTTTTGACAGTG | TGGATGCTCTCATCAGGACAG |
| Il-10 | GCTGGACAACATACTGCTAACC | GCATCACTTCTACCAGGTAAAAC |
| Il-6 | CCAGAGTCCTTCAGAGAGATAC | CTTATCTGTTAGGAGAGCATTGG |
| Lox | AGCTGTCACCAACATTACCAC | AGCTTGCTTTGTGGCCTTCA |
| Loxl1 | ACGTGCAGAGAGCCCATCTG | GGGAAGCGCAATAGCACTCG |
| Loxl3 | TGATGATGACTTCACGCTGCAG | AGATTGTCCAACCAGATTCGGC |
| Mcp1 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
| Plin1 | GGTACACTATGTGCCGCTTCC | CTTTGCGCTCCGCCTCT |
| Pparγ2 | TGCCTATGAGCACTTCACAAGAAAT | CGAAGTTGGTGGGCCAGAA |
| Prdm16 | TCCACAGCACGGTGAAGCCA | ATCTGCGTCCTGCAGTCGGC |
| TFIIβ | GTCACATGTCCGAATCATCCA | TCAATAACTCGGTCCCCTACAA |
| Tgfβ | CTCCCGTGGCTTCTAGTGC | GCCTTAGTTTGGACAGGATCTG |
| Tnfα | ATTCGAGTGACAAGCCTGTAGC | GGTTGTCTTTGAGATCCATGCC |
| Ucp1 | ACACCTGCCTCTCTCGGAAA | TAGGCTGCCCAATGAACACT |
| Atf4 | GTTGGTCAGTGCCTCAGACA | CATTCGAAACAGAGCATCG |
| Atf6 | TTATCAGCATACAGCCTGCG | CTTGGGACTTTGAGCCTCTG |
| Ire1 | CCCTGATAGGTTGAATCCTGGCTATGTG | AATCTATGCGCTAATCTGCT3GGCCTCTG |
| Xbp1s | GAGTCCGCAGCAGGTG | GTGTCAGAGTCCATGGGA |
| Chop10 | CTGCCTTTCACCTTGGAGAC | CGTTTCCTGGGGATGAGATA |
| Bax | TCCAGACAAGCAGCCGCTCA | TGCTGACGTGGACACGGACT |
| Pgc1-a | TCTCTGGAACTGCAGGCCTAAC | TCAGCTTTGGCGAAGCCTT |
| Ucp2 | GTTCCTCTGTCTCGTCTTGC | GGCCTTGAAACCAACCA |
| Ucp3 | AAGGATTTGTGCCCTCCTTTCT | AAAACGGAGATTCCCGCA |
| Ppara | CCTGAACATCGAGTGTCGAATATG | GCGAATTGCATTGTGTGACATC |
| Nrf1 | GAAACGGAAACGGCCTCATG | ACTCGCGTCGTGTACTCATC |
| Tfam | TCCCCTCGTCTATCAGTCTTG | AATTTGGGTAGCTGTTCTGTGG |
| Nox2 | CATCGGTGACAATGAGAACG | AAGGCCGATGAAGAAGATCA |
| Nox4 | ATCTTTGCCTCGAGGGTTTT | TGACAGGTTTGTTGCTCCTG |
| Ncf1 | TCCCTGCATCCTATCTGGAG | TCCAGGAGCTTATGAATGACC |
| Nos2 | GTTCTCAGCCCAACAATACAAGA | GTGGACGGGTCGATGTCAC |