Table 3.
Kinetic parameters of PAD-1, BKC-1, and KPC-2 against various β-lactam substrates.
| Substrate | PAD-1 | BKC-1 | KPC-2 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| K m(μM) | k cat(s−1) | k cat/K m (mM−1s−1) | K m(μM) | k cat(s−1) | k cat/K m (mM−1s−1) | K m(μM) | k cat(s−1) | k cat/K m (mM−1s−1) | |
| Oxacillin | 383.96 | 58.70 | 152.89 | 267.30 | 14,306.60 | 53,522.60 | NA | NA | NA |
| Cefazolin | 89.16 | 22.60 | 253.48 | NA | NA | NA | 16 | 44 | 2750 |
| Cefalotin | 91.59 | 15.20 | 165.96 | NA | NA | NA | 141 | 106 | 757.14 |
| Cephradine | 347.9 | 31.75 | 91.26 | NA | NA | NA | NA | NA | NA |
| Cefoxitin | ND | ND | ND | ND | ND | ND | 180 | 0.31 | 1.72 |
| Ceftazidime | 110.04 | 12.14 | 110.32 | 92.90 | 0.11 | 1.21 | ND | ND | ND |
| Cefoperazone | 47.80 | 15.08 | 315.48 | NA | NA | NA | NA | NA | NA |
| Ceftriaxone | 90.84 | 16.27 | 179.11 | 127.80 | 4.32 | 33.84 | NA | NA | NA |
| Cefotaxime | 137.08 | 20.06 | 146.34 | 223.90 | 0.46 | 2.08 | 174 | 66 | 379.31 |
| Cefepime | 108.40 | 10.4 | 95.94 | 174.30 | 1.69 | 9.69 | NA | NA | NA |
| Meropenem | 389.69 | 24.24 | 62.20 | 1.51 | 0.0034 | 2.25 | 15 | 4 | 266.67 |
| Aztreonam | 276.08 | 39.03 | 141.37 | 1,200.70 | 2.22 | 1.85 | 35 | 3 | 85.71 |