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. 2017 Aug 21;7:8871. doi: 10.1038/s41598-017-09011-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype analysis of cells recruited into the matrigel sponges by HmAIF-1 (A,B) and proliferation assay (C,D). After 1 week in vivo, MG is infiltrated by numerous cells stained by crystal violet and basic fuchsine (A). Ultrastructural analysis at TEM (B) confirm the macrophagic nature of the migrating cells in the MG sponges. Immunofluorescence experiment (C) shows BrdU⁺ nuclei (in red) in most of the cell migrated in the MG sponges. No signal is detected in negative control experiments (D) where the primary antibody was omitted. Nuclei are counterstained with DAPI (blue). Bar in (A) 50 µm; bar in (B) 2 µm; bars in (C,D) 10 µm.