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. 2017 Aug 21;7:8898. doi: 10.1038/s41598-017-09198-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Urocortin-1 regulates the expression of miRNAs in isolated cardiac myocytes through ERK1/2 and Epac2 activation. (A–C) Bar graphs show changes in the expression of miR-125a-3p (A), miR-324-3p (B) and miR-139-3p (C) in isolated cardiac myocytes treated with Ucn-1 (10 nM) in reperfusion. (D) Western blot and summary data showing ERK 1/2 phosphorylation in cells exposed to I/R (30 minutes/10 minutes). (E) Western blot and summary data showing Epac2 examined in cells exposed to I/R (30 minutes/18 hours each). “I/R” is for cells undergoing I/R protocols. “+Ast” is for cells subjected to I/R pre-treated 10 minutes with 0.5 µM astressin to inhibit CRF-R2 before the addition of Ucn-1. “+PD” is for cells pre-treated 10 minutes with 5 µM PD 098059 to inhibit ERK1/2, before the addition of Ucn-1. “+ESI-05” is for cells pretreated 10 minutes with ESI-05 (10 µM) to inhibit Epac2, before the addition of Ucn-1. “+8CPT” is for cells treated with 10 µM 8CPT at the onset of reperfusion. Values are shown in logarithmic scale and are means ± S.E.M, (n = 6–9 independent cells preparation). “*” and “**” indicate significance at p < 0.05 and p < 0.01.