Fig. 5.

Bs extracts Met from its neighboring Bm. a, b Bm (OS2) was pre-grown in the presence (a) or the absence (b) of Met (50 μg/ml), washed in PBSx1, mixed with Bs (LS5: ΔmetE) and resuspended in S7 supplemented with AHA (1 mM). Cells were incubated for additional 1.5 h, underwent click reaction and visualized by fluorescence microscopy. Shown are overlay images of fluorescence from AHA (green) and phase contrast (red; left panels), and AHA fluorescence alone (right panels). Fluorescence images were normalized to the same intensity range. c Bm (OS2), pre grown in the absence of Met, and Bs (LS5: ΔmetE) were mixed and incubated in the inner Transwell compartment. For a comparison, Bs (LS5: ΔmetE) was incubated in the outer Transwell compartment. Both the mixture in the inner well, and the Bs grown alone in the outer well, shared the same S7 medium containing AHA (1 mM). After 2 h of incubation, the cells underwent click reaction and visualized by fluorescence microscopy. Shown are overlay images of fluorescence from AHA (green) and phase contrast (red; left panels), and AHA fluorescence alone (right panels). Fluorescence images were normalized to the same intensity range. d Shown are CFU ratios at t _4-h/t ₀ of the following cultures: (1) Bs (LS5: ΔmetE) grown in the absence of Met. (2) Bs (OS21: ΔmetE, ΔymdB) co-cultured with Bm (OS2) in the absence of Met. (3) Bs (LS5: ΔmetE) co-cultured with Bm (OS2) in the absence of Met. (4) Bs (LS5: ΔmetE) grown in the presence of Met. Bm OS2 cells were pre-grown in the absence of Met. Shown are average values and s.d. of three independent experiments. Scale bars represent 10 µm