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. 2017 Aug 21;7:8341. doi: 10.1038/s41598-017-08823-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of RISC-Mediated Cleavage of the CLC-b-like and RPS3a-like mRNA by RLM RACE. (A) RPS3a-like mRNA/vd-sRNA duplexes predicted to be formed by the sRNA derived from the PSTVd variants. The arrows indicate the 5′ termini of RPS3a-like mRNA fragments isolated from the PSTVd-infected plants, as identified by 5′ RLM-RACE products, with the frequency of clones shown. (i.e. 17/20, indicates that 17 cleavage products were found out of 20 analyzed clones). Sequences are shown in the complementary polarity. The PARE reads were profiled against (B) the chloride channel protein CLC-b-like and (C) the RPS3a-like mRNAs. The vertical arrow denotes the predicted cleavage site. All of the reads were normalized to Transcripts Per Million (TPM). Note that different scales were used.