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. 2017 Aug 21;7:8870. doi: 10.1038/s41598-017-09200-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Arthritic TDAG8 knockdown mice show reduced mechanical hyperalgesia in the early phase, but TDAG8 knockout mice attenuated hyperalgesia in both early and late phases. (A) TDAG8 gene expression before (0 weeks) or after CFA injection (12 weeks) in L1-5 DRG. *P < 0.05, ***p < 0.001: shTDAG8-injected [(+)shTDAG8] vs vector-injected mice [(−)shTDAG8] by one-way ANOVA. (B–D) Arthritis scores (B) and joint diameter (C,D) in TDAG8-knockdown mice. *p < 0.05, ***p < 0.001: (+)shTDAG8 vs (−)shTDAG8 by two-way ANOVA. (E,F) PWT measured at weekly intervals after the first CFA injection. Data are mean ± SEM of total tested mice (n = 6 for (−)shTDAG8, and n = 8 for (+)shTDAG8). *p < 0.05, **P < 0.01, ***p < 0.001: (+)shTDAG8 vs (−)shTDAG8 by two-way ANOVA. (G,H) qRT-PCR analysis of ASIC3 or TRPV1 gene expression in ipsilateral L1-5 DRG of TDAG8 knockdown mice. Data are mean ± SEM. *p < 0.05, compared with 0 weeks; ^##p < 0.01, ^###p < 0.001, compared with WT control by one-way ANOVA. (I,J) PWT measured at weekly intervals after the first CFA injection in TDAG8^−/− or WT mice. Data are mean ± SEM of total tested mice (n = 4 for TDAG8^−/−, n = 6 for WT) *p < 0.05, **P < 0.01, ***p < 0.001: TDAG8^−/− vs WT by two-way ANOVA.