Figure 2.

Enrichment of ERF189/ERF199-binding P-box and MYC2-binding G-box elements in the promoter regions of structural genes involved in the nicotine biosynthesis pathway. Cis-regulatory P-box and G-box elements bound with cognate transcription factors in the promoter regions of nicotine biosynthesis genes are shown schematically at the top. As detailed in Kajikawa and Sierro et al. (2017), elements within 5′-flanking regions from –1,500 to –1 (numbered from the first ATG) were searched and scored using RSAT software (http://rsat.ulb.ac.be/rsat); elements with scores over 5.5 for P-boxes and 5.0 for G-boxes were adapted. Scores for each predicted element were summed for each gene promoter. Average values of the scores for each gene set (indicated below) are represented as black and gray bars with SDs for P-box and G-box elements, respectively. Student's t-test was used to calculate significant differences between values marked and those for genes involved in related primary pathways (left). **P < 0.01, *P < 0.05, n; numbers of genes involved in indicated gene sets.