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. 2017 Aug 16;10:289–294. doi: 10.2147/IMCRJ.S139729

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© 2017 Saksena et al. This work is published and licensed by Dove Medical Press Limited

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(A) 18S PCR results showing mixed infection inpatient samples. Lane 1 contains 100 bp ladder, lane 2 has Day 0 sample, and lane 3 has Day 3 sample. Both samples show mixed infections of P. falciparum at 205 bp and P. vivax at 120 bp, respectively. (B) RE analysis of pfcrt gene. Lane 1 contains Day 0 sample and lane 2 contains Day 3 sample with 50 bp ladder in lane 3. (C) Restriction digestion of pfmdr-1 amplicon. Lanes 1 and 2 are patient samples confirming the SNP (N86Y) in the amplicons (lanes 1 and 2 are Day 0 and Day 3 sample, respectively), lanes 3 and 4 are undigested amplicons, and lane 5 contains 100 bp ladder.

Abbreviations: PCR, polymerase chain reaction; RE, restriction enzyme; SNP, single-nucleotide polymorphism.